A statement from Duke President Vincent Price says the policy will cover all undergraduate, graduate, and professional students in all degree programs who intend to be on the Duke campus for any period of time starting with the fall semester.
Price says documented medical and religious exemptions will be accommodated.
The statement says students and employees who have not yet received a vaccination from Duke are urged to schedule an appointment as soon as possible.
Other universities including Brown, Cornell, Notre Dame, Northeastern and Rutgers have announced plans for similar vaccine requirements.
In March, Duke University enacted a stay-in-place order for all undergraduate students following a spike in COVID-19 cases that officials blamed largely on students attending fraternity rush events. In one week, more than 180 students were in isolation after testing positive, and another 200 students were in quarantine as a result of contact tracing.